Let $S \subseteq \overline{\mathbb{F}}_{p}$ be the set of roots of $x^{p^{n}} - x$. We first show that $S$ is a subfield of $\overline{\mathbb{F}}_{p}$, then we show that $\lvert S \rvert = p^{n} = \deg (x^{p^{n}} - x)$, this suffices to show that $x^{p^{n}} - x$ is separable.
First, $0 \in S$. Given $\alpha, \beta \in S$, we have $(\alpha - \beta)^{p^{n}} - (\alpha - \beta) = \alpha^{p^{n}} - \beta^{p^{n}} - (\alpha - \beta) = 0$. Hence $\alpha - \beta \in S \implies (S, +)$ is a subgroup of $(\overline{\mathbb{F}}_{p}, +)$. Next, $(\alpha\beta)^{p^{n}} - \alpha\beta = \alpha^{p^{n}}\beta^{p^{n}} - \alpha\beta = 0 \implies \alpha\beta \in S$ and $(\alpha ^{-1})^{p^{n}} - \alpha ^{-1} = (\alpha^{p^{n}})^{-1} - \alpha ^{-1} = 0 \implies \alpha ^{-1} \in S$ shows that $S$ is a subfield of $\overline{\mathbb{F}}_{p}$. Moreover, $\mathbb{F}_{p^{n}} = \mathbb{F}_{p}[S] = S$. This is because any $\alpha \in \mathbb{F}_{p}$ satisfies $\alpha^{p^{n}} - \alpha = \alpha^{p^{n} - 1}\alpha - \alpha = 1 \cdot \alpha - \alpha = 0$ by Fermat’s little theorem. Hence $\alpha \in S$ $\implies$ $\mathbb{F}_{p}[S] = S$.
Now since $(x^{p^{n}} - x)' = p^{n}x^{p^{n} - 1} - 1 = -1$, we have $\gcd(x^{p^{n}} - x, (x^{p^{n}} - x)') = 1$. By Proposition 1704, $x^{p^{n}} - x$ is separable over $\mathbb{F}_{p}$, hence $\lvert S \rvert = p^{n}$.
Then we prove the uniqueness of $\mathbb{F}_{p^{n}}$. Suppose that $F$ is a subfield of $\overline{\mathbb{F}}_{p}$ with $\lvert F \rvert = p^{n}$. Then the multiplicative group $F^{\times}$ is abelian of order $p^{n} - 1$. By Lagrange’s Theorem, we know that $\alpha^{p^{n} - 1} = 1$ for all $\alpha \in F^{\times}$. Hence every element in $F$ is a root of $x^{p^{n}} - x$, hence $F \subseteq \mathbb{F}_{p^{n}}$. Since they have the same cardinality, $F = \mathbb{F}_{p^{n}}$.